一株抗MRSA内生细菌的鉴定及其活性物质

Identification of endophytic bacteria KDNB6 from Sophora alopecuroides and antibacterial substance against MRSA

摘要:

根据生理生化特征和16S rDNA序列, 对一株从新疆苦豆子Sophora alopecuroides中分离的、对耐甲氧西林金黄色葡萄球菌(Methicillin-resistant Staphylococcus aureus, MRSA)具有较强拮抗作用的内生细菌菌株KDNB6进行鉴定; 并通过硅胶柱、凝胶柱层析从该菌发酵产物中分离纯化了抗MRSA的活性物质。结果表明菌株KDNB6各项特征与粘质沙雷氏菌Serratia marcescens最为接近, 鉴定为S. marcescens。从菌株KDNB6的代谢产物中分离到一个抗MRSA的活性成分。

Abstract:

An endophytic bacterium isolated from Sophora alopecuroides in Xinjiang, which had a conspicuous antagonistic effect on methicillin-resistant Staphylococcus aureus (MRSA), was studied and bioactive substances were separated and purified. Strain KDNB6 was identified as Serratia marcescens by its morphological, physiological and biochemical properties and 16S rDNA sequences. An anti-MRSA active component produced in fermentation broth was separated and purified by silica gel column chromatography and sephadex gel column chromatography.