洋葱伯克霍尔德菌T1828质粒消除方法及条件

Plasmid elimination method and conditions of Burkholderia cepacia T1828

摘要:

采用十二烷基硫酸钠和提高生长温度结合法、紫外线涂布平板法、冻融法、吖啶橙法和黄芩甙提取液消除法等方法消除洋葱伯克霍尔德菌T1828菌株质粒, 探讨适合洋葱伯克霍尔德菌质粒消除的方法, 并研究最佳质粒消除条件。结果表明十二烷基硫酸钠和提高生长温度结合法最适合于洋葱伯克霍尔德菌T1828质粒消除, 同时最佳消除条件为: 在T1828培养16 h后加入SDS使其终浓度0.1%, 36 °C处理18 h, 消除率达到49%。筛选到的无质粒突变株可以用于进一步的研究。

Abstract:

To explore the suitable for Burkholderia cepacia plasmid elimination method and study the optimal conditions for plasmid elimination, we eliminated plasmid of Burkholderia cepacia T1828 by using sodium dodecyl sulfate and improving the growth temperature combination method, UV-coated plate method, freeze-thaw method, acridine orange method and baicalin extraction methods such as elimination methods. The results showed that sodium dodecyl sulfate and improving the growth temperature combination method is best suited to Burkholderia cepacia T1828 plasmid elimination, while the best conditions for the elimination are T1828 culture after 16 h by adding SDS to 0.1% of the final solubility dealt with 18 h at 36 °C, the elimination rate reach 49%. The plasmid-free mutant strains can be used for further study.