β-葡萄糖苷酶新型高产菌株筛选及其液态发酵培养基的优化

Production of β-glucosidase by a novel isolated strain Tolypocladium cylindrosporum syzx4 and statistical optimization of fermentation media

摘要:

从腐败的玉米秸秆上分离得到一株能够发酵生产β-葡萄糖苷酶的弯颈霉属的柱孢弯颈霉菌株syzx4, 使用统计学方法对其发酵培养基进行优化。在单因子实验的基础上使用Plackett-Burman法对碳源、氮源和无机盐进行产酶显著性分析, 培养基组分对β-葡萄糖苷酶生产影响的排序为: 气爆秸秆粉(TCS)>KH2PO4>黄豆饼粉(SM)=(NH4)2SO4。响应面模型结果表明最优培养基为: TCS 25.72 g/L, SM 6.82 g/L, KH2PO4 1.90 g/L, (NH4)2SO4 3.21 g/L, 其余成分保持原始浓度。在30 °C发酵8 d, β-葡萄糖苷酶的活性可以达到2.21 U/mL。

Abstract:

A novel fungus, named Tolypocladium cylindrosporum syzx4, which can efficiently produce extracellular β-glucosidase, was isolated from naturally rotten corn stover. It is first time to report the β-glucosidase produced by T. cylindrosporum gams using agro-industrial residues in SmF. The fermentation variables optimized by single-factor experiment approach were further optimized by statistical optimization. Results of Plackett-Burman design indicated the evaluation of the medium components could be ranked as: TCS>KH2PO4>SM=(NH4)2SO4. With the optimum medium, viz. TCS 25.72 g/L, SM 6.82 g/L, KH2PO4 1.90 g/L and (NH4)2SO4 3.21 g/L predicted by RSM model with others as the original, 2.21 U/mL β-glucosidase activity was obtained. The results suggest that the β-glucosidase can be used for various biotechnological applications.