一株1,3-丙二醇生产菌的分离鉴定及其发酵特性

Isolation, identification and fermentation characterization of a 1,3-propanediol product bacterial

摘要:

从活性污泥中分离筛选得到一株能代谢甘油生产1,3-丙二醇(1,3-PD)的菌株2-1, 通过形态学鉴定、生理生化试验、16S rRNA序列分析对菌株分类学地位进行鉴定, 用MEGA 4.1软件构建的系统发育树显示菌株2-1与Klebsiella pneumoniae (CP001891)的亲缘关系最近。16S rDNA序列同源性比较发现, 菌株2-1与模式菌株同源率为95.4%, 疑似为新种。对菌株2-1在5 L发酵罐中进行发酵特性研究, 分批补料发酵时得到较高的1,3-PD终浓度, 达到63.5 g/L, 此时生产强度为2.19 g/(L·h), 底物转化率0.64 mol/mol。

Abstract:

A bacterial strain named as 2-1 was isolated from activated sludge, which can product 1,3-propanediol (1,3-PD) from glycerol. Morphologic properties, physiological and biochemical characteristics and 16S rRNA gene sequence analyses were used to identify the strain. The phylogenetic analysis of the 16S rRNA sequence indicated that the strain 2-1 closely with Klebsiella pneumoniae (CP001891), whereas, the homologous rate was 95.4%. The strain 2-1 might represent a new species. In the fed-batch fermentation at 5 L fermentor: the maximum concentration of 1,3-PD was 63.5 g/L; productivity was 2.19 g/(L·h); substrate conversion rate was 0.64 mol/mol.