灯台树内生放线菌多样性及抗菌活性评价

Diversity and antimicrobial activities of endophytic actinomycetes isolated from Alstonia scholar

摘要:

从云南西双版纳采集7份灯台树样品, 经过表面消毒, 用4种分离培养基分离得到105株内生放线菌。经16S rRNA基因序列分析鉴定, 分布于7个科9个属。利用5种植物病原真菌指示菌对所有菌株的发酵液进行抗菌活性检测。结果显示, 有12.4%、14.3%、11.4%、12.4%、8.6%的菌株分别对镰刀霉、疫霉、赤星霉、苹果炭疽、白色念珠菌有抗性。对3株具有广谱抗菌活性的菌株进行再次发酵和抗菌活性复筛, 结果显示这3株的抗菌活性稳定, 并可能含有生物碱类化合物。

Abstract:

One hundred and five endophytic actinomycetes were isolated using four different media from surface-sterilized tissues of 7 plants samples of Alstonia scholar collected from Xishuangbanna Yunnan province, southeast China. The results showed that they belong to 9 genera, 7 families by 16S rRNA gene sequences analysis. All actinomycete strains fermentation liquids were carried out the antimicrobial activities test against five plant pathogenic fungi. The results showed that 12.4%, 14.3%, 11.4%, 12.4%, 8.6% endophytic actinomycetes presented antimicrobial activity for Fusarium graminearum, Phytophthora nicotianae, Alternaria alternata, Colletotrichum gloeosporioides and Candida albicans, respectively. Three strains with strong antibacterial activities were refermented and rescreened, their antimicrobial activities were stability, and may produce alkaloids.