转化奎宁酮为(R)-3-奎宁醇菌株的筛选及转化条件优化

Isolation of a Rhodotourula mucilaginosa strain capable of transforming quinuclidinone into (R)-3-quinuclidinol and optimization of the transforming reaction conditions

摘要:

(R)-3-奎宁醇是一种用于合成各类药物的重要手性砌块, 以奎宁酮盐酸盐为唯一碳源, 筛选得到一株能够将奎宁酮不对称还原为(R)-3-奎宁醇的菌株X15。常规生理生化鉴定和18S rDNA序列分析表明, 菌株X15属于粘红酵母菌Rhodotorula mucilaginosa, 定名为R. mucilaginosa X15。结果显示, 菌株X15具有酮基还原能力和辅酶再生能力, 在100 mL反应体系中可将奎宁酮还原为(R)-3-奎宁醇, 转化率90%, ee值为88%。

关键词:奎宁酮酮基还原酶, 粘红酵母, 对称还原, (R)-3-奎宁醇

Abstract:

(R)-3-Quinuclidinol is an important chiral building block for the synthesis of a variety of pharmaceutical. A strain which is able to transforming quinuclidinone into reductase (R)-3-Quinuclid inol was isolated from soil by using quinuclidinone as sole carbon sourse in the screening medium. The physiological and bio-chemical identification and 18S DNA sequense analysis revealed that this strain belongs to Rhodotorula mucilaginosa and was named as R. mucilaginosa X15. The present results indicated that the optimal conditions for the transforming reaction with resting cells of R. mucilaginosa X15 were 3-quinuclidinone 10 g/L, pH 7.5, 30 °C and 72 h reaction time. (R)-3-Quinuclidinol is obtained with an yeild of 90% and with an optical purity of 88% enantiometric excess (ee) from a 100 mL reaction mixture.

Key words:3-Quinuclidinone reductase, Rhodotorula mucilaginosa, Asymmetric reduction, (R)-3-Quinuclidinol