旱地小麦根际细菌中产生1-氨基环丙烷-1-羧酸(ACC)脱氨酶菌株的分离和鉴定

Isolation and identification of wheat rhizosphere bacteria producing 1-aminocyclopropane-1-carboxylate (ACC) deaminase from drought soil

摘要:

采用富集定向筛选法, 从旱地小麦的根际土壤中分离到2株产生1-氨基环丙烷-1-羧酸(ACC)脱氨酶的菌株AS和CS。经测定菌株AS和CS的ACC脱氨酶的比活力分别为0.018 6 U/mg和0.016 7 U/mg蛋白。根据培养特征观察和生理生化指标测定结果, 结合16S rDNA碱基序列测定和系统发育同源性分析, 确定菌株AS和菌株CS分别属于霍氏肠杆菌(Enterobacter hormaechei)和变形斑沙雷氏菌(Serratia proteamaculans)。

关键词:ACC脱氨酶, 筛选, 鉴定, 根际细菌, 肠杆菌, 沙雷氏菌

Abstract:

Two bacteria strains marked AS and CS, producing 1-aminocyclopropane-1-carboxylate (ACC) deaminase, were isolated from wheat rhizosphere of drought soil. The activity of ACC deaminase from AS and CS strains was 0.018 6 U/mg and 0.016 7 U/mg protein, respectively. Based on the cultural morphological features as well as physiological and biochemical parameters in combination with 16S rDNA sequence analysis and sequence homology analysis, the strain AS and CS were identified as Enterobacter hormaechei and Serratia proteamaculans, respectively.

Key words:ACC deaminase, Screening, identification, rhizosphere bacteria, Enterobacter, Serratia