Abstract:To obtain the algicidal components from actinomycete strain L74, a series of techniques including centrifugation, ethanol precipitation, organic solvents extraction, silica gel adsorption chromatography, dialysis and chemical analysis were used. In addition, we studied the characteristic of stabilities of algicidal components under different pH and temperatures, and the influence of it to anti-oxidation enzyme system of Microcstis aeruginosa. The results suggested that the algicidal components may be or contain glycosides, lactones and triterpenes, which the molecular weights are less than 3.0 kD. In extraction experiments, petroleum ether, acetic ether and n-butanol can efficiently separate the algicidal components into water-layer. The algicidal components are stable for pH, but sensitive for temperature. After treated under 90 °C for one hour, the activity is nearly lost. When cultivated with algicidal components, the MDA contents of Microcstis aeruginosa increase and maintain at a high level, but the activities of SOD, POD and CAT significantly increase in the early cultivation and decline quickly in the late stage.
