利用响应面法优化ε-聚赖氨酸发酵培养基

Medium optimization of ε-polylysine production by response surface analysis

摘要:

采用响应面法对白色链霉菌ZC7发酵合成ε-聚赖氨酸的培养基进行优化研究。采用Plackett-Burman法对8个因素进行了筛选, 结果表明, 葡萄糖、酵母膏和硫酸铵的浓度对ε-聚赖氨酸产量影响较大。用最陡爬坡试验及Box-Behnken设计进一步优化, 利用Design-Expert软件进行二次回归分析, 得到各因素的最佳浓度为: 葡萄糖37.22 g/L, 酵母膏6.9 g/L, 硫酸铵6.55 g/L。在此优化条件下ε-聚赖氨酸的产量达到8.11 g/L, 较单因素试验最高值提高24.6%。

Abstract:

In order to enhance the concentration of ε-polylysine (ε-PL) with Streptomyces albulus ZC7, response surface methodology were used to optimize the fermentation medium. A Plackett-Burman design was used to evaluate the influence of eight factors firstly. Results showed that the concentration of glucose, yeast extract and (NH4)2SO4 played important roles in influencing the content of ε-PL. Then, the path of steepest ascent and the Box-Behnken design were adopted for further optimization, and the optimum concentration levels and the relationships among these factors was found out by quadratic regression model equation with Design-Expert statistic methods, the optimal concentration of the variables were determined as: glucose 37.22 g/L, yeast extract 6.9 g/L, (NH4)2SO4 6.55 g/L. Under such conditions, the concentration of ε-PL was increased to 8.11 g/L, which was 24.6% higher than the maximum value in the single factor tests.