活性炭对玉米芯半纤维素稀硫酸水解液的脱毒效果

Effect of the activated charcoal on corncob hemicellulose hydrolysate detoxification

摘要:

用稀硫酸对玉米芯半纤维素进行水解是一种常用的方法, 但是玉米芯半纤维素在水解成木糖等还原糖的同时还产生了糠醛、乙酸和酚类等抑制水解液发酵的毒物。以混合脱毒法为基础, 研究活性炭在脱毒过程中的作用。结果表明, 有脱毒效果的活性炭种类是GH-13和GH-15, 随着活性炭添加量的增大, 脱毒效果增强, 但木糖损失也随之增多。其中采用5% GH-15时的脱毒效果最佳, 该条件下乙酸去除率为24.60%, 糠醛去除率达100%, 酚类化合物去除效率R280值0.009, 而木糖的损失率为23.70%。

Abstract:

It is a common method that hemicellulose of corncob was hydrolyzed by dilute sulfuric acid to obtain hemicellulose hydrolysates. However, the hydrolysates of hemicelluloses contain not only xylose but also some inhibitors such as furfural, acetic acid and various phenol compounds. Based on the combination of multiple detoxification methods, the effect of activated charcoal in detoxification procedure was studied. The results showed that the activated charcoal GH-13 and GH-15 were effective for detoxification of the hydrolysate. The more the activated charcoal charge, the less the content of inhibitors in the hydrolysate, but the more xylose loss. GH-15 of 5% (W/V) was best for detoxification of the hydrolysate and acetic acid of 24.60% and furfural of 100% were removed in the condition, the R280 was 0.009, which stands for removal of phenolic compounds in hydrolysates, but xylose of 23.70% was lost in the process.