1,3-二氯苯对芦苇湿地土壤生物活性的影响

Influences of 1,3-dichlorobenzene on biological activity in reed wetland soil

摘要:

采用传统微生物培养及酶学方法, 研究了1,3-二氯苯(1,3-DCB)对芦苇湿地土壤微生物及酶活的影响。结果表明, 1,3-DCB处理芦苇湿地土壤对微生物不同类群影响不同, 其中对细菌和放线菌生长具有抑制作用, 各浓度1,3-DCB处理对放线菌的抑制较为显著, 浓度高于120 μg/g显著抑制对细菌的生长。1,3-DCB对真菌表现为先刺激后抑制。在实验过程中, 1,3-DCB可显著抑制湿地土壤过氧化氢酶和多酚氧化酶的活性, 表明这2种酶是1,3-DCB污染芦苇湿地土壤的敏感指标。

关键词:1,3-二氯苯, 微生物种群, 过氧化氢酶, 多酚氧化酶

Abstract:

With traditional microbiological culture and enzyme method, the effect of 1,3-dichlorobenzene (1,3-DCB) on reed wetland soil microbial and enzyme activities was studied. Results demonstrated that different groups of oil microorganisms have different responses to 1,3-DCB, in which the actinomycetes was significantly inhibited. When the 1,3-DCB concentration was higher than 120 μg/g, the bacterial amounts were significantly inhibited. The soil fungi amounts were initially increased at the beginning of the experiment but inhibited with time. During the experiment, 1,3-DCB treatment could significantly inhibit the soil catalase and polyphenol oxidase activity. Results indicated that these two enzymes are sensitive to 1,3-DCB contamination.

Key words:1,3-dichlorobenzene, Microbial community, Catalase, Polyphenol oxidase