毛云芝菌利用糖蜜酒精废水产漆酶培养基优化

Culture medium optimization for laccase production by Coriolus hirsutus using molasses distillery wastewater

摘要:

为提高毛云芝菌的漆酶产量, 降低生产成本, 同时使糖蜜废水中的营养成分得到充分利用, 变废为宝, 以糖蜜废水作为碳源, 对毛云芝菌发酵产漆酶进行研究。在单因素试验基础上, 以不同浓度糖蜜废水、尿素含量为自变量, 漆酶活力为因变量, 采用Box-Behnken设计方法得到最佳的培养基配方为糖蜜废水浓度47%, 尿素添加量0.5%, 漆酶活力可达1 810.0 U/mL, 约为优化前的6倍。说明毛云芝菌利用糖蜜废水作为碳源生产漆酶是可行的, 为糖蜜废水资源化利用高效生产有价值产品奠定了理论基础。

关键词:毛云芝菌, 漆酶, 糖蜜废水, 响应面法

Abstract:

In order to enhance the production of laccase and reduce the cost of laccase production, the culture medium for the growth of Coriolus hirsutus, a laccase production strain, was studied using molasses distillery wastewater (MDW) as carbon source. The different concentrations of molasses distillery wastewater and proportion of urea were optimized consequently through Box-Behnken experiment design under the foundation of signal factor experiment. As the optimization result, the production of laccase with the concentration of MDW 47% and proportion of urea 0.5% was six times higher than that from the original medium used previously. This indicates that the laccase production using MDW as carbon source is feasible. At the same time, the study makes the theoretical foundation for the exploitation and utilization of MDW to produce high value product.

Key words:Coriolus hirsutus, Laccase, Molasses distillery wastewater, Response surface method