蛹虫草表型多态性对子实体产生及虫草菌素的影响

The effect of polymorphism on fruiting body and cordycepin production of Cordyceps militaris

摘要:

为选育高产子实体和虫草菌素的蛹虫草菌株, 从野生蛹虫草中分离单孢子并进行分型,之后进行产子实体实验; 同时对蛹虫草子实体进行了产孢结构的观察, 并用高效液相色谱仪检测了子实体和培养基中的虫草菌素。结果表明, 菌落背面颜色为橙铬色的菌株容易产生子实体; 出发(原代)菌株所产子实体在形态上更接近野生蛹虫草, 而角变株的子实体畸形; 出发菌株产子实体能力要比该菌株未发生角变部分分离株和角变部分分离株都强。在虫草菌素的产量上也以出发菌株为高, 这表明表型多态性对蛹虫草产子实体和虫草菌素有很大的影响。本研究对于优势菌种的筛选和生产实践有借鉴意义。

关键词:蛹虫草, 表型多态性, 子实体, 虫草菌素

Abstract:

In order to screen and breed the strain with high fruiting body and cordycepin production, this study aims to investigate the effect of polymorphism on fruiting body and cordycepin production of C. militaris. Three progeny population were isolated from wild fruitbody of C. militaris, cultural characteristics of ascospores and fruitbody-producing ability were observed, and determined Cordycepin content in fruitbody and medium by HPLC. The results showed that the strains with colony reverse color of orange chrome could produce fruiting body easily. The sector mutation proportion of the strain produce few or no fruiting body. The shape and microstructure of fruiting body produced by original strain were similar to the wild strain in the nature. The original strain produced more fruiting body and cordycepin than the strains isolated from sector mutation proportion and the other parts without sector mutation. These results demonstrated the effect of polymorphism on fruiting body and cordycepin production of C. militaris.

Key words:Cordyceps militaris, Polymorphism, Fruiting body, Cordycepin