南极抗细菌活性菌株的筛选及系统发育分析

Screening and phylogenetic analysis of antibacterial strains isolated from antarctic

摘要:

分别以大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌、青枯假单胞菌、绿脓假单胞菌和苏云金芽孢杆菌为指示菌, 采用琼脂扩散法对实验室保存的580 株极地细菌进行了抗菌活性菌株的筛选与活性验证, 从中筛选出4 株对上述指示菌株具有明显抗菌效果的活性菌株, 其编号分别为97、Z11、Z18 及Z19, 并对其生长曲线、抗菌活性曲线和系统发育地位进行研究。结果表明, 4 株菌均在培养24 h 后进入指数生长期, 菌株97 在培养48 h 后达到稳定期, 而菌株Z11、Z18 及Z19在培养60 h 后达到稳定期。抗菌活性分别在培养84、96、72 和72 h 时达到最高。系统发育分析表明, 该4 株菌分别属于伦黑墨氏菌属(Rheinheimera)、嗜冷杆菌属(Psychrobacter)、假单胞菌属(Pseudomonas)和嗜冷杆菌属(Psychrobacter)。

Abstract:

Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas solanacearum, Pseudomonas aeruginosa and Bacillus thuringiensis were used as indicator strains respectively, four antibacterial strains, named as 97, Z11, Z18 and Z19 were screened from 580 strains of bacteria isolated from Antarctic by the method of agar diffusion. The growth curves, antibacterial curves and phylogenetic analysis of 4 antimicrobial strains were studied. The results showed that all of the four strains reached the log phase when they were cultured 24 h, strain 97 was in the log phase when it was cultured after 48 h, and the remaining three strains Z11, Z18 and Z19, were in the stationary when they were cultured after 60 h. They had the highest antibacterial activity when it was cultured after 84, 96, 72 and 72 h, respectively. Phylogenetic analysis indicated that these four strains belong to genera of Rheinheimera, Psychrobacter, Pseudomonas and Psychrobacter, respectively.