褐飞虱共生解脂假丝酵母抗吡虫啉菌株的驯化

Domestication for imidacloprid-resistant strain of rice brown planthopper, Nilaparvata lugens St?l, symbiote, Candida lipolytica

摘要:

为进一步研究共生菌在褐飞虱对吡虫啉产生抗性中的生理生化机制, 在稻田杀虫剂对褐飞虱共生解脂假丝酵母生长影响的基础上, 选用不同吡虫啉浓度进行抗药性菌株的驯化。结果表明, 褐飞虱共生解脂假丝酵母在不同吡虫啉浓度(2 000、1 000 和500 mg/L)的固体培养基上继代培养, 经过20 代后2 000 mg/L 培养基上的共生菌菌落数量, 与未加吡虫啉的培养基上的菌落数量差异不明显, 并且连续3 代稳定后定为抗2 000 mg/L 吡虫啉的共生菌菌株。在光镜下比较不同抗感吡虫啉菌株假菌丝的形态变化, 发现抗吡虫啉菌株的假菌丝出现畸形, 而且假丝变短, 部分出现了膨大。

Abstract:

To further study physiological-biochemical mechanism of symbiote in imidacloprid-resistant brown planthopper (Nilaparvata lugens), imidacloprid-resistant strain of symbiote, Candida lipolytica, was domesticated with different concentrations of imidacloprid based on effect of insecticides on growth and development of C. lipolytica. The results indicated that after in vitro C. lipolytica, on solid culture medium containing 2 000, 1 000 and 500 mg/L imidacloprid were subcultured for continuous 20 generations, the colony numbers of C. lipolytica on solid culture medium containing 2 000 mg/L imidacloprid were no significant difference with those on control medium containing non-imidacloprid. When numbers of colony kept stable for continuous three generations, the domesticated strain was called 2 000 mg/L imidacloprid- resistant one. Morphological changes of imidacloprid -resistant and -susceptible strains pseudohyphae were further observed using light microscope, it was found that pseudohyphae of imidacloprid- resistant strain occurred malformation and become shorter, some appeared enlargement.