一株产耐高温DNA 酶和蛋白水解酶的沙雷氏菌FS14 (Serratia sp. FS14) 的分离与鉴定

Isolation and chracterization of the Serratia sp. FS14 secreting thermostable DNase and protease

摘要:

通过组织分离法从白术病害样品的茎秆部位分离到一株产红色色素的细菌FS14, 参照《伯杰氏细菌鉴定手册》, 根据其形态学特征、生理生化特性, 同时结合16S rDNA 序列分析结果,发现该菌属于沙雷氏菌属。研究还发现, 从白术茎秆中分离到的这株中温型沙雷氏菌FS14 能分泌耐高温的DNA 酶和蛋白水解酶, 甚至在100 oC 预处理30 min 后仍有活性。沙雷氏菌能分泌耐高温的DNA 酶和蛋白水解酶还未见报道。

Abstract:

A red pigment producing bacterium strain FS14 was isolated from the stem of the diseased Atractylodes macrocephala Koidz. The strain was classified into Serratia according to its morphology, the physiological and biochemical features and 16S rDNA sequence. Different from the other reported Serratia species, the F14S strain could secret the thermostable DNase and protease. These secreted thermostable enzymes were still active even after pretreated at 100 °C for 30 min, while less activity was found after pretreated at 60 °C?70 °C for 30 min. The reason for this phenomenon need to be further investigated.