棉秸秆降解高温菌株的筛选及产酶分析

Isolation and characterization of four cellulose-decomposing thermophilic fungal strains from Xinjiang

摘要:

从新疆地区分离具有降解棉秸秆纤维素功能的菌株, 得到4 株耐高温真菌(50 °C)。纤维素酶学性质分析表明, 该4 株菌的纤维素酶具有良好的耐酸性(最适pH 为4.5)和耐高温性(最高达60 °C)。以羧甲基纤维素钠(CMC-Na)、微结晶纤维素、棉花、滤纸、淀粉、果胶为底物测定酶活力,滤纸酶活力(FPA)最高达2.63 U/mL、淀粉酶活力最高达6.17 U/mL、果胶酶活力最高达5.86 U/mL。4 株真菌酶学特性分析表明, 该系列菌株在秸秆生物质利用方面有很大的应用潜力。

Abstract:

Four thermophilic fungal strains producing cellulase (50 °C) were isolated from the compost of cotton residue in Xinjiang, having the ability of acid-resistant (the optimal pH is 4.5) and temperature-resistant (the highest temperature is 60 °C). The enzyme activities were measured using multiple substrates, such as sodium carboxy methyl cellulose (CMC-Na), microcrystalline cellulose, cotton, filter paper, starch and pectin. The highest filter paper enzyme activity (FPA) is 2.63 U/mL, the amylase activity is 6.17 U/mL and the pectinase activity is 5.86 U/mL. It was showed that these fungal strains and their secreted enzymes have great potential on cellulolytic biomass (cotton residue) degradation and utilization.