新疆泥火山细菌群落PCR-SSCP 分析

PCR-SSCP analysis bacterial community of Mud Volcano in Xinjiang

摘要:

采用单链构象多态性(SSCP)技术, 以16S rDNA 基因的V3 区为靶对象, 分析泥火山细菌多样性及其群落结构。通过对泥火山不同月份的不同深度土样DNA 提取后, 针对16S rDNA 进行PCR 扩增出236 bp 大小片段, 通过SSCP 电泳对泥火山细菌进行季节性多样性分析, 并对主要条带进行克隆分析。结果显示: 新疆泥火山细菌不同季节多态性明显, 而且这种多态性容易受生态和气候的影响; 假单胞菌属是泥火山土壤优势菌群, 在泥火山区土壤中广泛分布, 受生态和气候环境影响较小。

Abstract:

A technology sigle-strand conformation polymorphism (SSCP) and a target 16S rDNA gene on V3 region were used to analyze mud volcano microbial community and structure. After extracting DNA samples from different mud deepth and month, get a 236 bp fragment by PCR. The fragment was used to analyze the microorganisms seasonal diversity of mud volcano by SSCP, and some target fragments were analyzed clone. Resluts showed: the bacteria polymorphism in Xinjiang mud volcano soil is significant, which is vulnerable to ecological and climatics factors. Pseudomonas is a dominant microorganism in Xinjiang mud volcano, it distributes widely in mud volcano and was affected little by ecological and climatics.