海洋红酵母多糖提取及其对日本蟳血清中部分免疫活性因子的影响

Extraction of polysaccharide of oceanic red yeast and the study of it’s influence upon immunological activite enzymes of Charybdis japonica (A. Milne-Edwards)

摘要:

以海洋红酵母为材料, 通过化学抽提法得到多糖, 用经典的Sevag 法进行脱蛋白处理, 经多级沉淀得到纯糖并采用硫酸-蒽酮法测得其中葡萄糖含量; 考马斯亮蓝法分析蛋白质含量。以定量海洋红酵母多糖人工注射日本蟳, 注射等量生理盐水为对照, 定时测定其血清中部分免疫活性因子的活性; 实验表明: 提取多糖为蛋白多糖, 其中葡萄糖含量为3.6%, 蛋白质含量1.9%, 含有多种氨基酸, 其中天冬氨酸含量最多; 注射后12 h 日本蟳血清中总超氧化物歧化酶(T-SOD) 活力达到最高, 酸性磷酸酶(ACP) 活力在注射后24 h 达到最高, 碱性磷酸酶(AKP) 48 h 达到最高, 过氧化氢酶(CAT) 48 h 达到最高, 溶菌酶(LZM) 12 h 即达到最高, 最高点分别高于对照组24%、43%、25%、35%、95%; 72 h 后都恢复至对照组水平。结论: 海洋红酵母多糖注射48 h 内日本蟳体内免疫活性因子均有不同程度的提高, 对日本蟳有较强免疫刺激作用。

Abstract:

The polysaccharide from oceanic red yeast were purified using chemical extraction method. The classical Sevag mothed was carried out in order to deprotein and the pure sugar was obtained through multi stage precipitation. The content of glucose was determined by sulfuric acid anthrone method, and the content of protein was analysis by coomassie brilliant blue staining. After artificial infection by the quantitative polysaccharide of oceanic red yeast, the activities of the immunological activite enzymes in the serum of the Charybdis Japonica were measured periodically. Meanwhile, infection by the equivalent normal saline was served as control. Experiment show: The purified polysaccharide was proteoglycan. Including 3.6% of glucose and 1.9% of protein. It also contained many kinds of amino acids and the content of aspartate was the highest. After artificial infection, Total SOD (T-SOD) in the serum of the Charybdis japonica had maximum enzyme activity in 12 hours and acid phosphatases (ACP) in 24 hours, alkaline phosphatase (AKP) in 48 hours, catalase (CAT) in 48 hours, lysozyme (LZM) in 12 hours. The highest point was higher than those in the control group (24%, 43%, 25%, 35%, 95%). And the enzyme activities recovered to the corresponding control levels. Conclusion: The activities of the immunity active factors in the serum of the Charybdis Japonica have different degrees of the enhancement in 48 hours after infected with the polysaccharide of oceanic red yeast. The polysaccharide has stronger immune stimulation.