The taxonomy position and life cycles of culture-difficult fungi which associated with plants at various symbiosis extent are very completed and diverse. Most of these fungi can finish their life cycles when the other living things (such as plant, bacteria etc.) present. The non-obligate with no-vivo nutrition culture-difficult fungi are easier to be pure cultured than that of obligate with vivo nutrition one. On the basis of introducing taxonomy position, life cycle and symbiosis type of culture-difficult fungi associated with plants, the present author summarized the cultural characteristics and method for these fungi, as well as discussing the research trend and prospects, in order to provide new idea and basis for further investigating the pure culture of the fungi.