苦参碱对表皮葡萄球菌生物被膜作用初探

Preliminary study of effects of matrine on Staphylococcus epidermidis biofilms

摘要:

通过中药有效成分苦参碱对表皮葡萄球菌生物被膜抑制作用的研究, 目的是为表皮葡萄球菌生物被膜引起的相关感染提供新的治疗途径。方法是利用XTT减低法评价苦参碱对表皮葡萄球菌初始粘附及生物被膜内细菌代谢的影响, 镜下观察该药对表皮葡萄球菌生物被膜的形态学影响。结果是苦参碱对表皮葡萄球菌生物被膜菌的SMIC50和SMIC80分别为62.5 mg/L和500 mg/L; 1000 mg/L浓度的苦参碱对表皮葡萄球菌早期粘附有抑制作用; 250 mg/L浓度的苦参碱对表皮葡萄球菌生物被膜的形态有显著影响。结论是苦参碱对表皮葡萄球菌生物被膜的形成与粘附均有抑制作用。

Abstract:

A new therapeutic approach for Staphylococcus epidermidis biofilm-associated infections was provided by the study of active ingredient of Chinese medicine matrine on S. epidermidis biofilm inhibition. XTT reduction assay was used to evaluate matrine on the initial adhesion of S. epidermidis and biofilm metabolism, and microscope was applied to observe biofilm morphology. The results showed that SMIC₅₀ and SMIC₈₀ of matrine against S. epidermidis biofilms were 62.5 mg/L and 500 mg/L respectively, 1 000 mg/L of matrine inhibited the early adherence of S. epidermidis, and 250 mg/L of matrine had a significantly inhibitory effect on S. epidermidis biofilm morphology. The study demonstrated that matrine could inhibit S. epidermidis biofilm formation and adhesion.