PRRSV-GSLZ-1/2009细胞毒和组织毒致病特征的比较

Comparison of pathogenic characteristics between PRRSV-GSLZ-1/2009 cell propagated virus and field sample virus

摘要:

为了研究猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus, PRRSV)野毒株在细胞适应中的遗传变异与其致病性的关系。本文在进行遗传变异分析后, 将GSLZ-1/2009 细胞适应毒和组织毒同时接种36日龄健康仔猪, 对感染后的临床症状、体征变化、抗体及炎性因子水平进行了实时监测和动力学分析, 并对组织病变进行了显微观察。结果显示: 在细胞适应过程中致病相关基因并未发生任何突变, 但细胞适应株所诱发的抗体及炎症因子反应均明显较组织毒弱, 且所造成的病理损伤也比较轻微。表明PRRSV野毒株在细胞适应过程中毒力会减弱, 而毒株的弱化可能由遗传变异之外的因素决定。

Abstract:

The objective of this study was to analyze the correlation between genetic variation and pathogenicity of field sample virus during the propagation in sensitive cells. 36-day-old pigs were challenged with PRRSV GSLZ-1/2009 cell propagated virus and field sample virus after genetic variation analyse. Carry out real-time monitor and dynamics analyse to clinical symptoms, signs of change and the levels of antibody and inflammatory factors, and the histopathologic changes were observed under light microscope. The results showed that the disease-related genes was no variation, the antibody, inflammatory factors response and histopathologic lesions induced by field sample virus were more powerful than those induced by cell propagated virus. The researches indicated that virulence of field PRRSV could be weakened when it propagated in cells, and there are other possible factors except genetic variation playing on the role of virulence weaken.