培养法和免培养法联合检测油藏环境烃降解菌和产甲烷菌群多样性

Culture-dependent and culture-independent characterization of alkane-degrading bacteria and methanogens consortium diversity associated with petroleum reservoir

摘要:

烃降解菌和产甲烷菌是油藏环境微生物生态系统中重要的功能菌群, 采用DGGE和FISH方法分析了不同油藏样品中两类菌群的多样性和产甲烷活性。DGGE结果表明, 不同水样的alkB基因多样性相差较大, 而且注水井条带明显多于采油井。FISH结果表明, 油藏水样中产甲烷菌含量明显高于烃降解菌, 且两者空间分布的位置较近; 说明油藏环境中烃降解菌和产甲烷菌结成一定的相互关系。富集培养表明, 胜利油田产出液接种物培养130 d后, 石油烃降解率达到50%以上, 产甲烷的最大速率达到1.57×10^?2 mmol/(L?d)。利用分子生物学方法分析油藏环境功能菌群的多样性, 可以为开展微生物采油技术的应用提供有用信息。

Abstract:

The alkane-degrading bacterium and methanogenic were the important functional populations in oil reservoirs and their diversities were investigated by FISH and DGGE, respectively. The DGGE profiles showed great difference between the alkB genes in samples. And the major bands detected in injection wells were obviously more than them in production wells. The FISH results indicated that the quantity of archaea was much more than bacteria in the samples and they had close spatial distribution in situ. The results indicated that alkane-degrading bacterium and methanogenic populations were diverse in oil reservoirs and formed compact aggregates in situ. The incubation of Shengli oilfield production water degraded more than 50% residual oil during 130 d culture, with the production rate of methane reaching up to 1.57×10^?2 mmol/(L?d). Molecular analysis of the functional populations of microbial community in oilfield will provide the information for better application of microbial enhanced oil recovery.