野生鸡枞菌种长期保存方法比较

Comparison of Different Preservation Methods for Strains of Termitomyces

摘要:

野生鸡枞菌种质资源的有效保存是对野生鸡枞加以保护和利用的前提。以自行分离的5个野生鸡枞菌株作为研究对象, 采用蒸馏水保藏法和?80°C冻结保藏法对野生鸡枞菌种长期保存的方法进行了实验研究, 蒸馏水法分别保存于室温和4°C, ?80°C冻结保藏同时采用程控降温法和泡沫盒降温法, 保存20个月后对4种不同方法保存的5个菌株的保存效果进行比较。实验结果表明：蒸馏水室温保存法菌种存活率为100%, 萌发期较短, 为4?10 d, 是一种简便、实用、有效而成本低廉的长期保存方法; ?80°C冻结保藏法的存活率为56%?76%, 萌发期7?16 d, 泡沫盒降温法可以很好地控制降温速度, 是一种简便有效的控温方法。

Abstract:

Efficient storage of the wild germplasm is prerequisite for conservation and utilization of Termitomyces. In our study, using 5 isolated Termitomyces strains as materials, the preservation effects of distilled water preservation method and ?80°C freezing storage method were compared. These five wild strains of Termitomyces were stored in distilled water at room temperature and 4°C refrigerator, as well as preserved at ?80°C freezer for about 20 months after programmable cooler and styrofoam box controlled cooling. The survival rates of strains in distilled water were 100% after 20 months storage at room temperature, and that for frozen at ?80°C were 56%?76%. The strains preserved in distilled water at room temperature germinated in 4 to 10 d after inoculation, which is quicker than in ?80°C preservation (7?16 d). Styrofoam box method is an easy and effective method to control cooling speed compared to the programming method. In summary, preserving strains in distilled water at room temperature is a simple, practical and cheap method for long-term storage of Termitomyces germplasm.