嗜水气单胞菌佐剂灭活苗免疫中华鳖抵抗嗜水气单胞菌的致死性感染

Protecting Soft-shelled Turtle from Fatal Aeromonas Hydrophila Infection by Immunization with Aeromonas Hydrophila Emusion-oil Vaccine

摘要:

通过建立中华鳖浸泡感染模型评价中华鳖嗜水气单胞菌油乳剂灭活疫苗对中华鳖的免疫保护效力。在主动免疫保护试验中, 疫苗免疫组中华鳖能产生较高的抗体水平, 在使用10 × LD50的嗜水气单胞菌T3株进行浸泡攻毒后, 保护率为100% (10/10), 生理盐水对照组中华鳖的存活率仅为30% (3/10)。在被动免疫保护试验中, 疫苗腹腔免疫异育银鲫抗血清能80% (8/10)保护中华鳖抵抗10 × LD50的T3株的腹腔接种的攻击, 生理盐水对照组中华鳖的存活率为20% (2/10)。研究结果表明嗜水气单胞菌佐剂油乳剂灭活苗具有良好的免疫学原性, 可有效预防由嗜水气单胞菌引起的中华鳖红底板和肠道败血症等疾病。

Abstract:

We evaluated immune protection effectiveness of the aeromonas hydrophila emusion-oil vaccine in an immergence challenge model against haemorrhagic intestinal necrosis (HIN) and red abdominal shell by using soft-shelled turtles. In the active immune protection test, a high level of antibody was produced by the vaccine group. All the soft-shelled turtles in the vaccine group (10/10) were survived after challenged with 10 times 50% lethal dose (LD50) of virulent T3 strain, compared with 30% (3/10) survivors in the control group injected with 0.8% NaCl solution. In the passive immune protection test, 80% survivors (8/10) were observed in the group injected with serum, but only 20% (2/10) survivors were observed in the control group injected with 0.8% NaCl solution. These results showed that hydrophila Aeromonas emusion-oil vaccine had good immunogenicity, which could be used in the prevention of haemorrhagic intestinal necrosis (HIN) and red abdominal shell in soft-shelled turtles.