豆豉纤溶酶产生菌分离和鉴定

Screening and Identification of Douchi Fibrinolytic Enzyme-producing Strains

摘要:

从全国各地收集豆豉样品, 采用不同的培养基进行富集培养, 并利用纤维蛋白平板法高效获得了13株形态差异较大的产纤溶酶菌株。通过传统方法、化学方法以及16S rRNA序列分析对这13株菌进行分类鉴定, 它们分属于芽孢杆菌属、链霉菌属、假单胞菌属以及节杆菌属, 包括9种细菌, 丰富了豆豉纤溶酶产生菌菌种资源。

Abstract:

Various samples of Douchi, a kind of traditional Chinese fermented soybean food, were collected from provinces of China. Different strains with fibrinolytic activity were screened from these samples by enrichment culture medium and fibrin plate was used to confirm their fibrinolytic activity. Thirteen distinct strains were identified as Bacillus sp., Streptomyces sp., Pseudomonas sp. and Arthrobacter sp. by classical classification approaches, chemical approaches and 16S rDNA sequencing. Nine Dou chi fibrinolytic enzyme-producing strains were gained.