生姜根际茎腐病拮抗菌的筛选及鉴定

Isolation and Identification of Antagonist Bacteria from Ginger Rhizosphere Against Pythium myriotylum Drechsler

摘要:

从生姜根际土样分离获得的86个细菌分离物中, 通过离体拮抗试验, 筛选出2株对生姜茎腐病有良好拮抗效果的细菌, 编号分别为JF24和JF3。通过对它们进行形态学观察、生理生化测定以及16S rRNA序列分析, 初步确定, JF24为荧光假单胞菌(Pseudomonas fluorescens), JF3为粪产碱菌(Alcaligenes faecalis)。JF24和JF3的16S rRNA序列的GenBank登录号分别为FJ999730和FJ999731。

Abstract:

Eighty six bacterium isolates were obtained from rhizosphere of ginger. Through antagonistic tests, two antagonistic strains against Pythium myriotylum Drechsler were isolated, which were designated as JF24 and JF3, respectively. Based on the morphological, physiological and biochemical characteristics and phylogenetic analysis of 16S rRNA, JF24 and JF3 were identified as Pseudomonas fluorescens and Alcaligenes faecalis, respectively. The sequences of 16S rRNA of them have been submitted to GenBank and the accession numbers are FJ999730 and FJ999731, respectively.