Real-time PCR法检测日粮中添加小黑麦干酒糟对肉牛瘤胃Prevotella属菌数量的影响

Use of Real-time PCR Method in Determination of the Effects of Triticale Dried Distillers Grains with Solubles Inclusion in Beef Cattle Diets on the Populations of Rumen Prevotella Species

摘要:

通过在肉牛日粮中添加不同比例的小黑麦干酒糟及其可溶物(TDDGS), 运用Real-time PCR方法检测在添加TDDGS后对3种瘤胃普雷沃菌(Prevotella ruminicola、Prevotella brevis和Prevotella bryantii)数量的影响。结果表明, TDDGS组(20%、25%和30% TDDGS)与对照组(CG)相比, 瘤胃中P. ruminicola和P. brevis菌数量均有升高, 且在20% TDDGS组的数量分别显著升高47倍(P < 0.05)和794倍(P < 0.05), 而P. bryantii菌数量却有所降低, 并且在20% TDDGS组的数量显著降低5倍(P < 0.05); 另一方面, TDDGS组间相比, 除了P. ruminicola菌数量在20%和30% TDDGS组间存在显著差异, 其余TDDGS组间的3种菌数量差异均不显著。结论是在肉牛日粮中添加20% TDDGS对3种瘤胃普雷沃菌数量都产生了显著影响, 3种菌在TDDGS组间的数量变化差异不明显。

Abstract:

Using real-time PCR method to detect the effects of varying levels of triticale dried distillers grains with solubles (TDDGS) inclusion in beef cattle diets on the populations of three rumen Prevotella species viz., Prevotella ruminicola, Prevotella brevis and Prevotella bryantii. The results showed that 1) compared with control group (CG), the populations of P. ruminicola and P. brevis in all TDDGS groups (20%, 25% and 30% TDDGS) increased, and a 47-fold (P < 0.05) and a 794-fold (P < 0.05) significant increase were observed in 20% TDDGS group respectively, however, the population of P. bryantii decreased in all TDDGS groups and a 5-fold (P < 0.05) significant decrease was found in 20% TDDGS group; 2) compared among TDDGS groups, significant population difference was only observed between 20% and 30% TDDGS groups for P. ruminicola, however, no other significant population differences were found between the TDDGS groups for the three Prevotella species. The conclusion was that incorporation of 20% TDDGS in beef cattle diet significantly affected the populations of all three rumen Prevotella species but no obvious population changes were found between the TDDGS groups.