Mucoromycotina sp. HS-3对苯胺蓝染料的降解

Biodegradation of Aniline Blue Dye by a Fungus Mucoromycotina sp. HS-3

摘要:

采用静置开敞式培养法研究了碳源、氮源、盐度、金属离子对Mucoromycotina sp. HS-3菌降解苯胺蓝的影响。结果表明, 菌株脱色最适合条件为葡萄糖1 g/L, 硫酸铵0.6 g/L, Fe3+ 0.15 mmol/L, 盐度小于50 g/L, 在上述各培养条件下, 对浓度为100 mg/L不灭菌的苯胺蓝溶液静止培养5 d, 脱色率达95%以上。此外, 通过降解前后的苯胺蓝溶液对豇豆和枯草芽孢杆菌进行毒性测试发现, 降解后的苯胺蓝溶液毒性明显降低。因此, 该菌对处理以苯胺蓝为主要成分的印染废水具有较好的应用潜力。

Abstract:

An experiment on the effect of carbon source, nitrogen source, metal ion and salinity on degradation of aniline blue dye by Mucoromycotina sp. HS-3 was carried out under statically air-opened condition. The results showed that the optimum medium for single factor were 1 g/L glucose, 0.6 g/L ammonium sulfate, 0.15 mmol/L Fe3+, and less than 50 g/L salinity, respectively. Under each optimum condition above, the decolorization rate of aniline blue dye (100 mg/L initial concentration) was over 95% after 5 days. In addition, a toxicity test was carried out using a bioassay based on growth inhibition of Vigna unguiculata (a plant species) and Bacillus subtilis (a bacterial species) by aniline blue solution before and after degradation. The result showed that the toxicity of aniline blue after degradation had reduced obviously. So this strain had a good application potential for treating dyeing wastewater mainly consisted of aniline blue.