微生物学实验模块“蛋白酶产生菌的筛选、培养和选育”的教改研究与实践

A Experiment Module of Isolation, Cultivation and Breeding of Protease-producing Bacteria for Microbiological Experiment Teaching

摘要:

围绕“蛋白酶产生菌的筛选、培养和选育”主线, 设计了一个由5个相互关联、前后衔接、形成一体的实验组成的综合性研究型实验模块, 并进行教学实践。它们是: 培养基的配制、分装与灭菌; 产蛋白酶细菌的分离与纯化; 产蛋白酶细菌的培养发酵与条件优化; 产蛋白酶细菌的鉴定; 产蛋白酶细菌的紫外诱变育种。

Abstract:

We integrated a group of related experiments focusing on isolation, cultivation and mutation breeding of protease-producing bacteria into an experiment module and applied it in microbiological experiment teaching. The experiment module included preparation and sterilization of media, isolation of protease-producing bacteria, optimization of cultivation conditions, characterization of the strain, mutation breeding of the strain by UV exposure.