植物乳杆菌荚膜缺陷型菌株的诱变选育

Mutagenesis and Breeding of Capsule-deficient Lactobacillus plantarum

摘要:

以自主分离和鉴定的产荚膜多糖植物乳杆菌C88为出发菌株, 采用亚硝基胍诱变、墨汁负染和显微镜观察筛选获得一株荚膜缺陷型突变株, 命名为植物乳杆菌C88M3, 经多次传代突变菌株具有良好的遗传稳定性。通过16S rDNA序列分析、菌株生长曲线和RAPD分析比较了野生型菌株和荚膜缺陷型菌株在遗传特性和产荚膜情况方面的差异。通过化学诱变方法获得了乳杆菌荚膜缺陷型菌株, 对进一步研究荚膜多糖在乳杆菌益生性中的功能和作用机制具有重要意义。

Abstract:

The capsular polysaccharides-producing Lactobacillus strain isolated from traditional fermented products was used in this study. Using Lactobacillus plantarum C88 as an original strain for treatment with nitrosoguanidine and screening with negative staining, the capsule-de?cient mutant strain was obtained, it was named L. plantarum C88M3. On the basis of the results of 16S rDNA sequence analysis, growth test and RAPD analysis, compared with the wild type strain C88, the mutant strain C88M3 showed the difference in genetic characteristics and capsular polysaccharide production. In this paper, a capsule-de?cient mutant strain was obtained by inducing mutation methods, and the results of this work would provide further insight into the function and mechanism of probiotic action.