大气压辉光放电低温等离子体诱变选育谷氨酰胺转胺酶高产菌株

Mutation and Selection of Transglutaminase Producing Strain by Atmospheric Pressure Glow Discharge Plasma

摘要:

以茂源链轮丝菌(Streptoverticillium mobaraense) 03-10为出发菌株, 采用一种新型的裸露电极大气压辉光放电的冷等离子体技术对链霉菌孢子进行诱变。根据双层平板法菌落显色及诱变处理后菌落形态差异快速筛选谷氨酰胺转胺酶高产突变株。突变率、正突变率分别达到42.8%和20.6%。最后复筛选育出具有较好遗传稳定性和形态稳定性的高产突变株G2-1, 酶活达到2.73 U/mL, 比出发菌株提高了82%。

Abstract:

A novel mutation approach, namely, the atmospheric pressure glow discharge (APGD) plasma jet driven by a radio frequency (RF) power, was used to treat the spores of Streptoverticillium mobaraense 03-10 for the selection of transglutaminase (TGase) producer. The mutant with high TGase production was quickly screened according to the formation of color on the double-layered plate and the different appearances of colonies. The total mutation rate was over 42.8% and the positive mutant rate was 20.6%. The obtained mutant G2-1 has good genetic and morphological stability and TGase activity reached 2.73 U/mL, which was higher 82% than that of original strain.