辛辛那提弧菌选择性鉴别培养基的研制

Development of Vibrio cincinnatiensis Differential Medium

摘要:

旨在设计开发一种更适用于海产品重要致病菌——辛辛那提弧菌快速筛选与检测的选择性鉴别培养基(VciDM)。基于辛辛那提弧菌特有的综合酶系统及其代谢特点和对某些抑菌剂的抵抗力, 设计开发出一种VciDM, 用于辛辛那提弧菌的反复检验, 对VciDM的显色效果、平板效率、灵敏度和特异性进行评价。VciDM对辛辛那提弧菌具有较好的选择性和特异性; 在37°C ± 0.1°C培养16?24 h, 辛辛那提弧菌菌落为黄色; 其他致病菌菌落呈绿色或不生长; VciDM对辛辛那提弧菌的出菌率高, 为(82.14 ± 4.45)%; VciDM的检出限为101 CFU/mL; 与常规方法相比, 结果易于观察, 减少了溶藻弧菌等常伴弧菌的干扰, 省去了TCBS分离培养后的生化试验, 降低检测成本, 减少工作量, 具有更高的检测效率和鉴别能力。VciDM平板可以对辛辛那提弧菌进行快速监测。

Abstract:

To develop a more economical, simple and rapid detecting and differentiating Vibrio cincinnatiensis Differential Medium (VciDM), V. cincinnatiensis is one species of the major pathogens in seafood. Based on the specific enzyme systems and the corresponding metabolisms of V. cincinnatiensis, according to the resistibility to different antibacterial ingredients, VciDM was designed for the rapid separation, identification or detection of V. cincinnatiensis. The tests of color effects, plating efficiency, sensitivity and specificity were done to evaluate the efficiency of VciDM. The results showed that: VciDM had good selectivity and specificity. After incubation for 16?24 h at 37°C ± 0.1°C, the V. cincinnatiensis formed yellow colonies on VciDM. The other species of bacteria did not form colonies or formed green colonies on VciDM. VciDM showed a mean plating efficiency of V. cincinnatiensis cells of (82.14 ± 4.45)% and its detection limit was 101 CFU/mL. As compared with the conventional methods, VciDM used for isolating and detecting V. cincinnatiensis, which is simpler operation, lower cost, more convenient for observation, higher sensitivity and specificity. Accordingly, it is suitable for kinds of inspection agencies to detect V. cincinnatiensis, especially for the use in rapid detecting and monitoring V. cincinnatiensis in seafood in the primary production and processing plants. VciDM can be used to rapidly and initiatively detect V. cincinnatiensis, which is usefull to rake quick infection control measurees.