一株产阿魏酸酯酶青霉菌株的筛选、鉴定及生长特征

Isolation, Identification and Growth Characteristics of a Penicillium Strain Producing Feruloyl Esterases

摘要:

从腐烂的木质纤维中筛选了一株产阿魏酸酯酶的菌株HDFE1, 根据其形态特征、rDNA ITS1-5.8S-ITS2序列及系统发育分析, 鉴定菌株HDFE1为青霉属的橘青霉(Penicillium citrinum Thom)。菌株HDFE1最适生长温度为30°C, 最适生长pH 为6.0。该菌株在30°C、pH 6.0、200 r/min培养60 h时, 阿魏酸酯酶酶活力为最高, 达20.75 U/L。

关键词:rDNA ITS1-5.8S-ITS2区, 阿魏酸酯酶, 鉴定, 生长特性

Abstract:

The strain HDFE1, producing feruloyl esterases, was isolated from rotten wood fiber. It was identified as Penicillium citrinum Thom by study of morphology and rDNA ITS1-5.8S-ITS2 sequence and phylogenetic analysis. The optimum conditions for its growth were pH 6.0 and 30oC. At 30oC, pH 6.0, and 200 r/min for 60 h, the strain could produce feruloyl esterases at a yield 25 of 20.75 U/L.

Key words:Feruloyl esterases, rDNA ITS1-5.8S-ITS2, Identification, Growth characteristics