一株以葡萄糖为底物产2,3-丁二醇微生物菌株的筛选及鉴定

Screening and Identification of a Strain Producing 2,3-Butanediol from Glucose as Substrate

摘要:

从自然界中筛选出一批以葡萄糖为底物发酵产2,3-丁二醇的菌株, 经初步发酵测定发酵液中2,3-丁二醇含量, 其中菌株6-7的2,3-丁二醇产量最高达49.6 g/L。对其进行常规生理生化鉴定实验, 并结合16S rDNA序列分析, 比对结果表明, 菌株6-7与Bacillus subtilis strain BIHB332相似性达99%。在细菌分类学上属于枯草芽孢杆菌属, 将其命名为Bacillus subtilis 6-7。其特点是属于环境友好和食品安全型菌株, 因此, 利用Bacillus subtilis 6-7生产2,3-丁二醇具有良好的工业应用价值。

Abstract:

Most strains with capability of producing 2,3-butanediol (2,3-BDO) from glucose were isolated from different natural environment samples. The Strain 6-7 with high capability of producing 2,3-BDO was screened from the isolated strains and the production of 2,3-BDO reached 49.6 g/L. In addition to general morphological and biochemical characteristics, the strain 6-7 was identified by 16S rDNA sequence and systematic analysis. The results showed that 16S rDNA sequence of strain 6-7 had similarity of 99% with Bacillus subtilis strain BIHB332, suggesting that the strain 6-7 is one of Bacillus subtilis species. It is an environmentally friendly strain and Bacillus subtilis is generally regarded as safe microorganism used for industrial production. This strain showed the potential application in the future.