一种简捷的玉米大斑病病菌单孢分离方法

A Simple and Feasible Method for Single-spore Isolation of Exserohilum turcicum

摘要:

介绍一种利用普通光学显微镜直接分离玉米大斑病病菌单孢子的方法。该方法是通过敲打叶片先将孢子转移至水琼脂培养基上, 然后利用自制的简易挑孢针在低倍镜下直接挑取大斑单孢子, 从而达到分离纯化的目的。结果表明, 此法虽然有一定的操作难度, 但简便易行, 污染少, 高效快捷, 为玉米大斑病病菌生理小种鉴定、DNA遗传多态性的进一步研究做了积极有益的基础工作。该方法值得推广到其他种类大型孢子真菌的单孢分离工作中。

Abstract:

This paper introduces a method for single-spore isolation of Exserohilum turcicum with general light microscope. Spores are displaced from the corn leaf to Water Ager by tapping, single-spore of Exserohilum turcicum are then picked with simple homemade needle at low magnification so as to achieve purification. Although the method was slightly sophisticated, it was simple, practical, less contaminating and highly effective. The application of the method is contributive to rapid identification of physiological races and DNA genetic polymorphism of Exserohilum turcicum. It is more suitable for single-spore isolation of the pathogenic fungi with large conidiospores.