RNA干扰(RNAi)文库研究进展

Progress in Construction of RNAi Library for Generation of siRNAs

摘要:

RNAi是由双链RNA (dsRNA)引发的转录后基因沉默现象, 由dsRNA产生的小分子siRNA会导致生物体内同源转录产物特异性降解, 是基因表达调控的重要方式之一。目前RNAi技术已发展成为遗传分析强有力的工具, 在基因功能分析鉴定方面发挥越来越大的作用。构建大规模的RNAi文库进而转变成RNAi突变体库是功能基因组学研究的重要手段, 因此如何利用简单经济的方法构建特定物种的高效RNAi文库就成为关键问题。综述了目前构建RNAi文库的不同方法以及每种构建方法的优点和存在的不足, 为不同研究目的的RNAi文库的构建提供参考。

Abstract:

RNA interference (RNAi) is a post-transcriptional gene silencing mechanism that is triggered by double-stranded RNA (dsRNA) and results in the degradation of homologous transcripts. RNAi technology has been developed as a powerful tool for genetic screens to analyze gene function in various organisms. Construction of large-scale species-specific RNAi library and further RNAi mutant library is important strategies for functional genomics research. So how to construct RNAi libraries simply and efficiently is becoming a key question in gene function research. In the review, representative strategies for RNAi library construction and their advantages and potential disadvantages were summarized.