海带配子体克隆中一株镰刀菌的分离鉴定

Isolation and Identification of a Fusarium sp. from Laminaria Gametophyte Clones

摘要:

从海带配子体克隆中分离出一株真菌(菌株编号: 059601016C), 对其培养性状、形态特征和ITS基因进行研究分析。结果显示: 059601016C真菌在PDA培养基上呈棉絮状生长, 菌落背面颜色由白色变为深紫色。气生菌丝发达, 高度可达5 mm-7 mm。小型分生孢子以链状或假头状着生于瓶状产孢细胞上, (5.0?10.5) μm × (1.2?2.5) μm。大型分生孢子镰刀状, 略有弯曲, 顶胞渐尖, 2?5个隔膜, 多3?4个隔膜。通过ITS基因序列同源性分析, 该菌株与层出镰刀菌的相似性为100%。系统发育树的分析结果也表明该菌株与层出镰刀菌的亲缘关系最接近, 因此将菌株059601016C鉴定为层出镰刀菌(Fusarium proliferatum Nirenberg)。在GenBank中申请的基因序列号为GU951805。

Abstract:

A fungal strain (No. 059601016C) was obtained from Laminaria Gametophyte Clones. Growth states, morphological characteristics and internal transcribed sequence (ITS) were studied. The results showed that colonics on PDA are white and cottony, the reverse color changed from white to dark purple. The aerial mycelium grew abundantly and the height reached 5 mm to 7 mm. Microconidia are in false head or chains, (5.0?10.5) μm × (1.2?2.5) μm. Macroconidia sickle-shaped were slightly bent, apex acuminate, 2?5 septate, mostly 3?4 septate. Based on the analysis of ITS and the phyogenetic tree, the strain showed a 100% homogeneous and the most close relationship to Fusarium proliferatum Nirenberg. Hence, the strain 059601016C isolated form Laminaria Gametophyte Clones belonged to the genus Fusarium sp. and the GenBank accession number of strain 059601016C ITS is GU951805.