原生质体诱变选育ε-聚赖氨酸高产菌株

Microbial Breeding of High ε-polylysine-producing Strains by Diethyl Sulfate Protoplast Mutagenesis

摘要:

以白色链霉菌UN2?71为出发菌株, 对其原生质体进行硫酸二乙酯(DES)诱变, 选育ε-聚赖氨酸高产菌株。经过试管初筛和摇瓶复筛, 得到1株稳定性好的菌株D3-32, 摇瓶产量达到1.56 g/L, 比出发菌株提高49.43%。采用2.3 L发酵罐进行发酵试验, 控制pH分两阶段培养后, ε-聚赖氨酸最高产量达到4.59 g/L, 比出发菌株提高了2.65倍。

Abstract:

Streptomyces albulus UN2?71 was selected as original strain and its protoplast was treated by diethyl sulfate (DES) to obtain high ε-polylysine producing mutants. Through primary screening and confirmed screening with shake flask cultures, one strain designated as D3-32 with a good genetic stability has been acquired, whose production in the shake flask reached 1.56 g/L, which was about 49.43% higher than that of original strain. In the fermentation test with 2.3 L fermentor, after a pH-controlled two-phase fermentation, the highest production of ε-polylysine reached 4.59 g/L, which was increased by 2.65 times compared to that of the original strain.