培养和非培养法分析冷藏鸡肉胴体中的细菌多样性

Bacterial Diversity in Chilled Chicken by 16S rDNA-ARDRA and Culture Dependent Approach

摘要:

运用纯培养和非培养法对冷藏鸡肉胴体上细菌多样性进行了对比研究。采用培养法从鸡肉胴体中初步分离到45株细菌菌株, 16S rDNA-ARDRA分析得到9株代表性细菌, 其16S rDNA序列系统发育分析表明, 这些菌株隶属于Bacillus sp.、Shigella sp.、Pseudomonas sp.、Citrobacter sp.、Klebsiella sp.和Escherichia sp. 6个属。16S rDNA-ARDRA联合PAGE和16S rDNA全序列分析的非培养法结果表明, 冷藏鸡肉中细菌主要属于Acinetobacter sp.、Bacillus sp.、Acidovorax sp.、Brochothrix thermosphacta、Lactococcus garvieae和Leuconostoc lactis等16个属。非培养法揭示的细菌多样性比培养法丰富, 但二者结合使用能让肉品中微生物多样性得到更全面的展示。

Abstract:

Bacterial diversity of chilled chicken was investigated by culture independent and dependent approach. A total of 45 colonies were recovered from the plate culture of chilled chicken based on morphological characteristics and further identified as 6 genera, including Bacillus sp., Shigella sp., Pseudomonas sp., Citrobacter sp., Klebsiella sp. and Escherichia sp., according to their 16S rDNA sequences. Direct 16S rDNA-ARDRA coupled with sequences analysis of DNA extracted from chilled meat showed that the bacteria could be assigned to 16 genera of Acinetobacter sp., Bacillus sp., Acidovorax sp., Brochothrix thermosphacta, Lactococcus garvieae and Leuconostoc lactis etc. Compared to culture dependent method, culture independent approach disclosed a more diversified bacterial community in chilled chicken, but culture dependent one was still a necessary supplement by showing unique species when molecular analysis was used to investigate microbial diversity.