杀线虫放线菌DA09202的鉴定及其活性物质的解析

Identification of Nematicidal Actinomycetes DA09202 and Active Compounds

摘要:

从海南热带植物园土壤样品中分离获得一株具有较强杀线虫活性的放线菌菌株DA09202, 通过形态特征、生理生化特征、16S rDNA序列测定及其系统发育分析, 初步鉴定为金色链霉菌。菌株DA09202发酵液采用溶媒萃取、硅胶柱层析、Sephadex LH-20凝胶过滤和制备薄层板层析, 从中分离得到杀线虫活性化合物A23-1和A46-2。化合物A23-1经光谱和波谱分析(UV、1H-NMR、13C-NMR、DEPT、1H-1H COSY、HMBC、HSQC)以及文献对照, 鉴定为4￠,7-二羟基异黄酮, 化合物A46-2的结构正在鉴定中。

Abstract:

Strain DA09202 with nematicidal activity was isolated from soil sample collected from Hainan Tropical Botanical Garden. Based on the mycelium morphology, physiological property, biochemical property and 16S rDNA phylogenetic tree, strain DA09202 was identified as streptomyces aureus. The results indicated that the strain DA09202 has nematicidal activity, the active compounds A23-1 and A46-2 were purified from its fermentation broth by means of solvent extraction, column chromatography, Sephadex LH-20 and preparation TLC, and the structure of the A23-1 was elucidated as 4￠,7-Dihydroxyisoflavone by spectrum analysis, and the structure of A46-2 is been elucidating.