一株甲烷氧化菌的分离鉴定与特性

Isolation, Identification and Characterization of Methanotrophic Bacteria

摘要:

甲烷氧化菌是一类能以甲烷作为唯一碳源和能源进行同化和异化代谢的微生物。从若尔盖高原不同地点采集的样品中筛选得到一株名为XN1的甲烷氧化菌, 根据此菌株的形态与16S rRNA序列同源性分析, 证实该菌株属于Methylomonas属。对该菌株的培养条件进行研究的结果表明, 以甲烷与甲醇共同作为碳源, 硝酸钾和氯化铵共同为氮源时菌生长最好, 最适生长温度为25°C, 最适生长pH为6.5, 培养基中CuSO4·5H2O和FeSO4·7H2O的浓度以0.03 mg/L和0.4 mg/L为宜。

Abstract:

Methanotrophs are a kind of bacteria which can use methane as the sole carbon source and energy for their anabolism and catabolism. A strain methanotroph was isolated from different samples of the Zoige Plateau, named XN1, and was studied. It was identified a strain of Methylomonas through 16S rRNA sequencing and comparison. The investigation result showed that the optimal conditions for the strain were methane gas together with methanol as carbon source and the coexistence of NH4Cl and KNO3 as nitrogen source, the cultivation temperature of 25°C and medium initial pH of 6.5, and the optional concentrition of CuSO4·5H2O of 0.03 mg/L and FeSO4·7H2O 0.4 mg/L.