玉米秸秆青贮过程中优势细菌多样性分析

Diversity of Predominant Bacteria During Ensiling

摘要:

通过从发酵0、1、3、5、15、30和60 d自然发酵的青贮玉米(CK)和采用菌剂处理的青贮玉米(处理1)上取样, 分别进行pH值测定和菌群总DNA的提取。总DNA经纯化后采用引物341F-GC和518R进行细菌16S rDNA V3区PCR扩增, 扩增产物进行变性梯度凝胶电泳(DGGE)分离并回收优势条带和变化明显的条带进行测序分析, 了解秸秆饲料发酵过程中的优势细菌多样性及动态变化规律, 从而为现有青贮接种菌的改良奠定理论基础。研究结果表明: 采用菌剂处理的青贮玉米pH值下降更快, 其优势细菌种类更丰富, 且各优势菌的丰度比对照组中相应菌的丰度更强。

Abstract:

To analyze the diversity and dynamic changes of predominant bacteria during ensiling, the silage fermented in natural and silage treated with inoculants,which named CK and unit 1 respectively, were sampled in the case of days 0, 1, 3, 5, 15, 30, 60. The pH of each sample was measured and the genomic DNA of microorganism was extracted respectively. After purified by DNA gel Recovery kit, the genomic DNA was subjected to PCR with 16S rDNA (V3 region) primers (341F-GC and 518R). The amplified DNA fragments were separated by denaturing gradient gel electrophoresis (DGGE) and the dominant bands were excised for sequence analysis. The results showed that the pH of unit 1 dropped more rapidly than that of CK, the species of predominant bacteria and numbers of each species also more abundant.