TEMPO/STA法在水产品金黄色葡萄球菌计数检测中的应用

Application of TEMPO/STA Method for Enumeration of Staphylococcus aureus in Aquatic Products

摘要:

金黄色葡萄球菌能够引起细菌性食物中毒, 其对水产品的污染将严重影响到食品安全和水产品加工出口贸易。本研究使用TEMPO/STA法和PetrifilmTM测试片法对535个出口水产品样品进行检测, 并将检测结果进行比较。结果表明, TEMPO/STA法与PetrifilmTM测试片法一致性较好(符合率96.6%, 准确度无差异), 并具有操作简单、快速、准确和人为误差小的特点。

Abstract:

Staphylococcus aureus can cause alimentary toxicosis, contamination of which could exert a great influence on food safety and export processing of aquatic products. In this study, TEMPO/STA method was used for enumeration of Staphylococcus aureus in 535 aquatic samples, and compared with PetrifilmTM Staph Express Count Plate method. TEMPO/STA method was demonstrated to be a rapid and accurate method, with a good agreement (96.6% compliance rate, no difference in accuracy) with the other one.