1. Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China; 2. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong 510070, China 在期刊界中查找 在百度中查找 在本站中查找
1. Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China; 2. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong 510070, China 在期刊界中查找 在百度中查找 在本站中查找
1. Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China; 2. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong 510070, China 在期刊界中查找 在百度中查找 在本站中查找
1. Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China; 2. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong 510070, China 在期刊界中查找 在百度中查找 在本站中查找
1. Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China; 2. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong 510070, China 在期刊界中查找 在百度中查找 在本站中查找
Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that amplified DNA with high specificity, efficiency and rapidity under isothermal conditions using a set of four specially designed primers and a DNA polymerase with strand displacement activity. The cycling reaction continues with accumulation of 109-1010 copies of target in less than an hour. The final products are stem-loop DNA with several inverted repeats of the target and cauliflower-like structures with multiple loops. A positive reaction would be shown as a ladder-like pattern in a gel electrophoresis analysis. Because of its simplicity, high sensitivity and specificity, the LAMP method has been widely applied to the field of food safety inspection.
