1. Huazhong Agricultural University of Life Science and Technology, Wuhan, Hubei 430070, China; 2. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agri 在期刊界中查找 在百度中查找 在本站中查找
In order to obtain genomic DNA which meets the requirements of DNA-DNA hybridization, CTAB method combined with liquid nitrogen grinding was investigated. Cultures from different media added with glycine were harvested in different time. Quality of extracted genomic DNA was assessed by electrophoresis and OD260/OD280 value. Mycelia of shaking culture in yeast extract & malt extract (YE) medium containing 0.3% glycine for 3 days were subjected for DNA extraction. The genomic DNA obtained was about 20 kb, with the OD260/OD280 value around 1.8.
