一株产蛇孢假单壳素真菌F02Z2172的形态和分子鉴定

Morphological and Molecular Identification of Ophiobolins Producing Strain F02Z2172

摘要:

从厦门武夷山自然保护区土壤样品中分离得到一株可产生蛇孢假单壳素(Ophiobolins)的丝状真菌, 编号为F02Z2172。通过对其宏观特征、显微特征进行观察并对其ITS、Calmodulin和β-tubulin序列进行分子系统学分析, 鉴定为伪弯头曲霉, 并对伪弯头曲霉与焦色组内其他菌种的关系进行了讨论。由伪弯头曲霉产生蛇孢假单壳素还是首次报道。

关键词:蛇孢假单壳素, 伪弯头曲霉, 转录间隔区, 钙调素基因, β-微管蛋白基因, 焦色组

Abstract:

A filamentous fungus F02Z2172 which is able to produce ophiobolins was isolated from Wuyishan Nature Reserve in Fujian province. The strain was identified as Aspergillus pseudodeflectus by cultural and morphological characteristics and phylogenetic analysis based on ITS rDNA, Calmodulin and β-tubulin sequences. The relation between A. pseudodeflectus and other species of Aspergillus Section Usti was also discussed. This was the first report of ophiobolins produced by A. pseudodeflectus.

Key words:Ophiobolins, A. pseudodeflectus, ITS, Calmodulin, β-tubulin, Aspergillus Section Usti