向日葵菌核病拮抗菌XRK5的筛选与鉴定

Isolation and Identification of Antagonistic Bacteria Inhibiting Against Sclerotinia sclerotiorum from Sunflower Rhizosphere

摘要:

从向日葵根际分离了640个细菌分离物, 以向日葵菌核病菌(Sclerotinia sclerotiorum)为靶标菌, 通过平板对峙法获得了18个具有拮抗性能的细菌, 其中XRK5具有较强拮抗能力, 且拮抗性能稳定, 具有较好的生防应用潜力。经过形态观察、生理生化特征及16S rRNA 序列分析, 将XRK5鉴定为辣椒溶杆菌(Lysobacter capsici), XRK5的16S rRNA序列在GenBank中的注册号为FJ959348。

关键词:向日葵菌核病, 拮抗细菌, 鉴定

Abstract:

We isolated 640 isolates from sunflower rhizosphere. Eighteen antagonistic bacteria screening have against Sclerotinia sclerotiorum. Isolate XRK5 has obviously and steady antibiosis against the pathogenic fungus, and it implys that XRK5 has good application potentiality. Based on the results of morphological characteristics, physiological and biochemical properties and phylogenetic analysis of 16S rRNA, XRK5 was identified as Lysobacter capsici. The sequence of 16S rRNA obtained in this study has been submitted to GenBank and the accession number is FJ959348.

Key words:Sclerotinia sclerotiorum of sunflower, Antagonistic bacteria, Identification