MALDI-TOF质谱技术对克罗诺杆菌的鉴定与分型

Identification and Typing of Cronobacter spp. by MALDI-TOF MS

摘要:

以基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)技术用于克罗诺杆菌的鉴定与分型。通过对获得的克罗诺杆菌属典型菌株、阴沟肠杆菌和产气肠杆菌近似菌株以及克罗诺杆菌分离株的蛋白质质量图谱进行对比分析, 找出克罗诺杆菌特征性离子峰, 将其作为鉴定克罗诺杆菌的生物标识物; 对全细菌蛋白质质量图谱进行聚类分析, 将克罗诺杆菌属进一步划分为不同类型, 结果显示, 4株克罗诺杆菌参考菌株质量图谱约在5740 (m/z)离子质荷比处出现1个相近离子峰, 28株克罗诺杆菌分离株中27株(占96.4%)表现出相同结果; 32株克罗诺杆菌被分为6种类型(以50%距离水平为分类界限)。MALDI-TOF MS 作为一种新的技术, 不仅能够用于克罗诺杆菌的鉴定, 而且根据获得的细菌蛋白质质量图谱可将克罗诺杆菌划分为不同类型。

Abstract:

Identification and typing of Cronobacter spp. was performed by matrix-assisted laser desorption/ionisation time of flight mass spectrometry (MALDI-TOF-MS). Protein mass spectrometric profiles obtained from reference strains and isolates of Cronobacter spp. and Enterobacter cloacae, Enterobacter aerogenes were compared and analyzed, characteristic peak at about 5740 (m/z) was selected as biomarker for identification of Cronobacter spp., 27 of 28 isolated strains could be identified by this marker. Cluster analysis of whole protein mass spectrometric profiles of 32 Cronobacter spp. strains shown that Cronobacter spp. could be divided into 6 different types. MALDI-TOF MS can be used as a new technology for identification as well as typing of Cronobacter spp. based on the protein mass spectrometric profiles.