氯嘧磺隆降解菌的分离鉴定及其降解特性

Isolation, Identification and Degradation Characteristics of Chlorimuron-ethyl Degradation Bacteria

摘要:

利用富集培养技术从长期施用氯嘧磺隆的土壤中分离得到1株能够降解氯嘧磺隆的细菌L-7。通过生理生化特性和16S rRNA序列分析, 初步鉴定L-7为寡养单胞菌属(Stenotrophomonas sp.)。并分析了氯嘧磺隆的初始浓度、接种量、温度和pH值对L-7菌株降解氯嘧磺隆效果的影响,确定了最佳降解条件。结果表明, 该菌在氯嘧磺隆浓度为100 mg/L、接种量为5%、pH 4.0、温度30°C条件下, 接种5 d后对氯嘧磺隆的降解效率达到80%以上。

Abstract:

L-7, a bacterium which was able to degrade chlorimuron-ethyl, was isolated from the soil of long term applied with chlorimuron-ethyl by enrichment culture. Base on physiological and biochemical characteristics and 16S rRNA sequence analysis, the strain L-7 was identified preliminarily as Stenotrophomonas sp.. The effect of initial concentration of chlorimuron-ethyl, inoculation amount, temperature and pH on degradation efficiencies was studied. The optimal degrading conditions were: initial concentration of chlorimuron-ethyl 100 mg/L, inoculation amount of 5%, pH 4.0, respectively, under the optimal conditions, the degrading efficiency could reach more than 80% after 5 days at 30°C.